WebSep 15, 2024 · Here we report CRY2–CIB1 and CRY2–CRY2 interactions are governed by well-separated protein interfaces at the two termini of CRY2. N-terminal charges are … WebFeb 2, 2024 · To spatially, temporally, and cell-type-specifically modulate brain circuits, optogenetic strategies have emerged. The light-gated cation channel channelrhodopsin-2 (ChR2) is used widely for stimulating brain networks by depolarizing membrane potential followed by illumination ( Boyden et al., 2005; Lin et al., 2009; Nagel et al., 2003 ).
Spatiotemporal control of intracellular phase transitions using …
WebJan 12, 2024 · We use this "optoDroplet" system to study condensed phases driven by the IDRs of various RNP body proteins, including FUS, DDX4, and HNRNPA1. Above a concentration threshold, these constructs undergo light-activated phase separation, forming spatiotemporally definable liquid optoDroplets. WebJan 12, 2024 · Rapid Light-Dependent Clustering of IDR Fused Cry2 (A) Schematic diagram of the optogenetic platform. Three IDR-containing RNA binding proteins are used in this … list of writing genres for ks2
Optogenetic control of cellular forces and mechanotransduction
WebSep 15, 2024 · CRY2 variants with different oligomerization propensities offer an additional layer of optical control for biological processes, as demonstrated using an opto-Raf system to tune the levels of Raf/MEK/ERK signaling. We envision that CRY2high will be a useful tool for optical strategies that require robust CRY2 oligomerization. Moreover, as the ... WebFeb 10, 2024 · CRY2-mCherry, CIBN-GFP-CAAX were gifts from Chandra Tucker (Denver, Colorado, United States) 30. The DHPH domain of ARHGEF11 Guanine Exchange Factor (gene ID 9826, also known as PDZ-RhoGEF) was ... WebActivation of endoplasmic reticulum stress via clustering of inner nuclear membrane proteins. bioRxiv, 2024. Optogenetic activators of apoptosis, necroptosis and pyroptosis for probing cell death dynamics and … imo car wash freiberg