Phospho-ampk substrate motif
WebTable: PTMScan®Results,Phospho-AMPK Substrate Motif (LXRXXS*/T*), SILAC Study design: Human non-small cell lung cancer (H1650) cell line; LysC/post-IAP trypsin digest; Antibody: Phospho-AMPK Substrate Motif (LXRXXS*/T*), PTMScan®Kit ##5564 Treatments: Untreated (Light), Phenformin-treated (Heavy) 2 WebPTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit Description: PTMScan® Technology employs a propri-etary methodology from Cell Signaling Technology for …
Phospho-ampk substrate motif
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WebAMPK to undergo a conformational change, exposing an activation loop of the a subunit, allowing phosphorylation on Threonine172 by upstream kinases, referred to as AMPK … WebWe systematically explored the effects of the AMPK-MFN2 signaling axis on dynamic changes of mitochondria, autophagy and MAMs in response to energy stresses. Results Energy stress promotes MAM formation, autophagy and mitochondrial fission
WebApr 7, 2024 · AMPK is rapidly activated following any stress that triggers a lowering of the intracellular adenosine 5′-triphosphate (ATP)/adenosine 5′-diphosphate (ADP) ratio, … WebFeb 6, 2024 · The phosphorylation of S461 in the presence or absence of cisplatin was measured by an antibody recognizing phospho-AMPK substrate motif [LXRXX(pS/pT)].
WebPhospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ™ Rabbit mAb mix. Western blot was imaged using Odyssey ® Infrared Imaging System … WebDec 23, 2010 · Many in vivo substrates of AMPK not only conform to this motif but also bind to the phospho-binding protein 14-3-3 inducibly upon phosphorylation by AMPK. We therefore screened for proteins that bound to recombinant 14-3-3 in wild-type (WT) but not AMPK-deficient cells, and only under conditions of energy stress, when AMPK would be …
WebMar 13, 2024 · The first, based on the AMPK phosphorylation site of ACC at Ser79, was the development of a specific peptide substrate (the SAMS peptide) that could be used to measure AMPK activity 336.
WebApr 11, 2024 · In LNCaP cells, inhibition of GSK3β by LiCl led to increased phosphorylation of its substrate glycogen synthase (pGS ... TPD52 characteristic D2-motif, PEST sequences, ... The results showed that the AICAR an activator of AMPK by phosphorylation significantly downregulated TPD52 expression in both LNCaP and VCaP cells. While investigating ... pineway golf clubWebJan 10, 2024 · The phosphosites that were upregulated in WT cells but did not meet these rules and did not have sequences similar to the conserved AMPK substrate motif constituted group 2 ( n = 77). These sites may be regulated by the AMPK-dependent signaling pathways but are not directly phosphorylated by AMPK. lea michele famous birthdaysWebDec 3, 2024 · Introduction. 5' AMP-activated protein kinase (AMPK) family members share a high degree of sequence similarity within the N-terminal kinase domains [], but their regulatory C-termini are far more divergent.Phosphorylation of a conserved threonine residue within the activation loop is necessary for the activation of all AMPK family … lea michele films et tvWebFeb 16, 2024 · A previous quantitative phospho-proteomics study uncovered an overall comprehensive regulatory role of AMPK during DDR with multiple newly identified substrates (Chen et al., 2024), while the intact role and related mechanism of AMPK in DSBR remained unknown.TALEN (transcription activator-like effector nuclease) technology was … pineway golf course lebanonWebPhospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The … lea michele fired funny girlWebNov 3, 2015 · To characterize the AMPK network, we conducted a screen to identify direct AMPK substrates and phosphorylation sites. We used an analog-specific (AS) method of kinase-substrate identification (Banko et al., 2011, Shah et al., 1997), which utilizes a mutant of AMPK that accepts N 6-(phenethyl) ATPγS, a bulky form of ATP introduced into cells … lea michele ellen showWebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes were incubated with ... lea michele full lyric